Bulgaria Microbiome R&D Center
Research
GB BIO SCIENCE research team presents a new technology and concept, which demonstrate that NIR spectroscopy and Aquaphotomics when applied for differentiation of closely related microorganisms with different phenotypic characteristics provide very accurate, fast and non-invasive identification of probiotic strains based on spectral monitoring data of bacterial growth.
We were the first to use this innovative method for in-vivo evaluation of lactobacilli and selection of probiotic strains with predefined characteristics.
Research Articles
Investigation of the antimicrobial activity of polyphenol-enriched extracts against probiotic lactic acid bacteria
April 20, 2020
The antimicrobial activity of polyphenol-enriched extracts from industrial plant by-products (strawberry and bilberry press residues and distilled rose petals) against probiotic lactic acid bacteria (Lactobacillus delbrueckii subsp. bulgaricus S10 and S19; Lactobacillus rhamnosus YW and S25; Lactobacillus gasseri S20; Streptococcus thermophilus S13 and S32) was investigated.
Selection of Lactobacilli with Probiotic Propertiesria
April 20, 2020
The aim of the present work was to select strains from genus Lactobacillus with properties,allowing their inclusion in probiotics and probiotic foods - survival in model conditions ofdigestion, high antimicrobial activity and resistance to some of the applied in medicinal treat-ment antibiotics.
The Efect of the Immobilization of Probiotic Lactobacilli in Chitosan on their Tolerance to a Laboratory Model of Human Gut
April 20, 2020
A successful immobilization in chitosan of the lactic acid bacteria Lactobacillus acidophilus A., Lactobacillus helveticus H., Lactobacillus casei subsp. casei C. and Lactobacillus plantarum 226-15 was accomplished and high viable cell counts the gel beads were obtained. The resistance of the included in the gel matrix cells to model conditions of digestion was investigated.
The Efect of the Immobilization of Probiotic Lactobacilli in Chitosan on their Tolerance to a Laboratory Model of Human Gut
April 20, 2020
A successful immobilization in chitosan of the lactic acid bacteria Lactobacillus acidophilus A., Lactobacillus helveticus H., Lactobacillus casei subsp. casei C. and Lactobacillus plantarum 226-15 was accomplished and high viable cell counts gel beads were obtained. The resistance of the included in the gel matrix cells to model conditions of digestion was investigated.
Probiotic Strain Lactobacillus Plantarum NBIMCC 2415 with Antioxidant Activity as a Starter Culture in the Production of Dried Fermented Meat Products
April 20, 2020
Lactobacillus plantarum NBIMCC 2415 resistant to low and high values of pH and pepsin, and to high concentrations of bile salts was selected. It was demonstrated that Lactobacillus plantarum NBIMCC 2415 inhibited the growth of pathogenic microorganisms such as Escherichia coli ATCC 25922, Escherichia coli ATCC 8739, Proteus vulgaris G, Salmonella sp., Salmonella abony NTCC 6017, Staphylococcus aureus ATCC 25093, Staphylococcus aureus ATCC 6538 P, Listeria monocytogenes and Listeria monocytogenes I at temperature 15-18 C in the meat products.
Investigation of antimicrobial activity of polyphenol-enriched extracts against probiotic lactic acid bacteria
April 20, 2020
The antimicrobial activity of polyphenol-enriched extracts from industrial plant by-products (strawberry and bilberry press residues and distilled rose petals) against probiotic lactic acid bacteria (Lactobacillus delbrueckii bulgaricus - S10 and S19; Lactobacillus rhamnosus - YW and S25; Lactobacillus gasseri - S20; Streptococcus thermophilus - S13 and S32) was investigated.
Application of Aquaphotomics to learn more about the rules of water
April 20, 2020
All living creatures are made of cells,small membrane-bounded compartments filled with a concentrated aqueous solution of chemicals
Rapid bacteria selection using Aquaphotomics and near infrared spectroscopy
April 20, 2020
Efficient, quick and inexpensive screening methods, which provide rapid, comprehensive probiotic bacteria evaluation, are highly desired in contemporary microbiology. Aquaphotomics is a novel scientific approach for the exploration of aqueous systems through rapid and comprehensive analysis of water-light interaction as a potential source of information for better understanding of the biological world using spectroscopy.
Monitoring of Water Spectral Pattern Reveals Differences in Probiotics Growth When Used for Rapid Bacteria Selection
April 20, 2020
Development of efficient screening method coupled with cell functionality evaluation is highly needed in contemporary microbiology. The presented novel concept and fast non-destructive method brings in to play the water spectral pattern of the solution as a molecular fingerprint of the cell culture system.
Monitoring of water spectral patterns of lactobacilli development as a tool for rapid selection of probiotic candidates
April 20, 2020
Nowadays a quick and inexpensive method, which allows rapid, in vivo comprehensive probiotic bacteria identification, is needed. To elucidate a new concept to evaluate probiotic bacteria, near infrared spectroscopy with aquaphotomics were applied to monitor the growth of eight Lactobacillus bulgaricus and one Lactobacillus gasseri bacteria strains.